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KMID : 0363620060270020171
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Journal of Korean Oriental Medicine
2006 Volume.27 No. 2 p.171 ~ p.181
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Peroxynitrite Scavenging Activity of Vespae Nidus
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Jung Ji-Chun
Shin Won-Young
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Abstract
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Objectives :Peroxynitrite(ONOO^-), superoxide anion({cdot}{O_2}^-) and nitric oxide (NO) is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities for ONOO^- and its precursors, NO and {cdot}{O_2}^- of Vespae Nidus.
Methods :Dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used to investigate scavenging activities of ONOO^-,;NO,;{cdot}{O_2}^-. Six-months-old ICR mice were used. After mice were injected with lipopolysaccharides(LPS), kidney organization was evaluated. Three comparison groups of ICR mice were used : a normal group, an experimental group that was fed Vespae Nidus extract and then injected with LPS, and a control group that was injected with LPS. Scavenging activities of ONOO^-,;NO,;{cdot}{O_2}^- in these groups were measured in the same way.
Results :Vespae Nidus markedly scavenged authentic ONOO^-,;{cdot}{O_2}^- and NO. It also inhibited ONOO^- induced by {cdot}{O_2}^- and NO which are derived trom SIN-1. Furthermore, it inhibited $ONOO^-,;{\cdot}{O_2}^-$, and NO generation by Vespae Nidus in LPS-treated ICR mouse kidney postmitochondria.
Conclusions :These results suggest that Vespae Nidus might be developed as an effective ONOO^-,;{cdot}{O_2}^-, and NO scavenger for the prevention of the aging process and age-related diseases.
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KEYWORD
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Vespae Nidus, peroxynitrite, nitric oxide, reactive oxygen species
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